The cDNA sequence of human NAMPT was amplified by PCR from a pGex-6p-three-hNAMPT plasmid (kindly gifted from Dr Shui-qing YE, College of Missouri). The PCR solutions have been digested and cloned into a pET21a+ vector employing NdeI and XhoI restriction enzymes. Some extent mutation was introduced by the fast transform web page-directed mutagenesi
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Activated AMPK acutely triggers the destruction of existing defective mitochondria through ULK1-dependent mitophagy and at the same time triggers the biogenesis of recent mitochondria by using effects on PGC-1a dependent transcription. These twin processes controlled by AMPK possess the Internet effect of replacing existing faulty mitochondria with